Journal: iScience
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
doi: 10.1016/j.isci.2025.112198
Figure Lengend Snippet:
Article Snippet: Guide sequences (non-targeting – nt-sgRNA-1: 5′- GCGGGCAGAACGACCCTGAC -3', nt-sgRNA-2: 5′- GAAGACGTGCTGGCGTCACC -3'; ANXA4-targeting – ANXA4-sg1: TCTGCTGGCTATAGGTAAGC(TGG), ANXA4-sg2: GGACGATGCTCTCGTGAGAC(AGG) - (PAM sequence in brackets)) were cloned into TLCV2-LoxP backbone (TLCV2-LoxP was a gift from Adam Karpf (Addgene plasmid #127098; http://n2t.net/addgene:127098; RRID:Addgene_127098).
Techniques: Virus, Generated, Recombinant, Membrane, Electron Microscopy, Saline, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Western Blot, RNA Sequencing, Gene Expression, Imaging, Plasmid Preparation, shRNA, Control, Luciferase, Cloning, Expressing, Software, Cell Analysis, Microscopy, Cell Culture